Open AccessIdentification of some bacterial cell components by FT‐IR spectroscopy *
Author(s) -
Helm Dieter,
Naumann Dieter
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07393.x
Subject(s) - dipicolinic acid , endospore , bacilli , bacterial cell structure , bacteria , infrared spectroscopy , clostridia , bacillus (shape) , fourier transform infrared spectroscopy , chemistry , spectroscopy , biochemistry , biology , microbiology and biotechnology , spore , organic chemistry , genetics , chemical engineering , physics , quantum mechanics , engineering
Some particular cell components can be detected and identified by Fourier Transform infrared spectroscopy (FT‐IR) of intact bacteria. Typical marker bands were used to identify these bacterial cell components. Polypeptide capsules were detected in several Bacillus species by a band typical for α‐helical structures and by strong carboxylate stretching vibrations. Formation of endospores in clostridia and bacilli was discovered using marker bands for dipicolinic acid. Spectra of some Bacillus strains showed expression of poly‐β‐hydroxybutyric acid granules, capsules and endospores simultaneously.