Open AccessThe effect of carbohydrates on the expression of the Prevotella ruminicola 1,4‐β‐D‐endoglucanase *
Author(s) -
Gardner Richard G.,
Wells James E.,
Russell James B.,
Wilson David B.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07373.x
Subject(s) - xylanase , xylose , biochemistry , cellulase , chemistry , arabinose , xylan , glycoside hydrolase , cellobiose , hydrolysis , enzyme , fermentation
The β‐1,4‐endoglucanase of the ruminai bacterium, Prevotella ruminicola B 1 4, hydrolysed carboxymethylcellulose and barley glucan but not xylan or mannan. Endoglucanase activity was present in 88‐ and 82‐kDa proteins, and there was at least a 20‐fold variation in endoglucanase activity when P. ruminicola B 1 4 was grown on different sugars. The highest activities were observed with mannose, cellobiose or xylose and little activity was observed with sucrose, arabinose or rhamnose. P. ruminicola B 1 4 also had significant xylanase and mannanase activities, but these activities were present in proteins that had lower molecular masses than the endoglucanase and these proteins did not cross‐react with antibody made against the endoglucanase. Mannanase activity has a similar pattern of expression to the endoglucanase, while the xylanase was not induced or repressed by the same sugars or combinations of sugars. The xylanase activity was greatest when xylan was the energy source for growth, but xylose was a very poor inducer of xylanase activity.