Open AccessTransformation of Aspergillus nidulans by microprojectile bombardment on intact conidia
Author(s) -
Fungaro Maria Helena P.,
Rech Elibio,
Muhlen Gilda S.,
Vainstein Marilene H.,
Pascon Renata C.,
Queiroz Marisa V.,
PizziraniKleiner Aline A.,
Azevedo João Lúcio
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07371.x
Subject(s) - aspergillus nidulans , transformation (genetics) , auxotrophy , plasmid , conidium , biology , dna , microbiology and biotechnology , genetics , gene , chemistry , escherichia coli , mutant
This paper describes transformation of intact conidia of Aspergillus nidulans , auxotrophic for arginine, by using the biolistic process. The plasmid employed was pFB39, carrying the argB gene. The transformation frequency obtained was 81 transformants/ μg of DNA. Classical genetics and molecular analysis were conducted to analyse transformants and to determine in which chromosome integration took place.