Open AccessCloning and sequencing of the pac gene encoding the penicillin G acylase of Bacillus megaterium ATCC 14945
Author(s) -
Martín Laura,
Prieto María A.,
Cortés E.,
García JoséL.
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07370.x
Subject(s) - bacillus megaterium , open reading frame , escherichia coli , biology , nucleic acid sequence , biochemistry , cloning (programming) , gene , molecular cloning , nucleotide , peptide sequence , leucine , microbiology and biotechnology , enzyme , amino acid , bacteria , genetics , computer science , programming language
The pac gene encoding the penicillin G acylase (PGA) of Bacillus megaterium ATCC 14945 has been cloned in Escherichia coli HB101 ( proA, leuB ) using a selective minimal medium containing phenylacetyl‐L‐leucine instead of L‐leucine. The nucleotide sequence of this gene has been determined and contains an open reading frame of 2406 nucleotides. The deduced amino acid sequence shows significant similarity with other β‐lactam acylases. Although the PGA of B. megaterium is extracellular, the enzyme produced in E. coli appears to have a cytoplasmic localization.