Open AccessIsolation and characterization of a new hydrogen‐utilizing bacterium from the rumen
Author(s) -
RieuLesme Françoise,
Fonty Gérard,
Doré Joël
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07338.x
Subject(s) - rumen , fermentation , bacteria , biology , yeast , fructose , biochemistry , microbiology and biotechnology , food science , chemistry , genetics
A new H2 CO2‐utilizing acetogenic bacterium was isolated from the rumen of a mature deer. This is the first report of a spore‐forming Gram‐negative bacterial species from the rumen. The organism was a strictly anaerobic, motile rod and was able to grow autotrophically on hydrogen and carbon dioxide. Acetate was the major product detected. Glucose, fructose and lactate were also fermented heterotrophically. The optimum pH for growth was 7.0–7.5, and the optimum temperature was 37–42 °C. Yeast extract was required for growth and rumen fluid was highly stimulatory. The DNA base ratio was 52.9 ± 0.5 mol% G + C. On the basis of these characteristics and fermentation products, the isolate was considered to be different from acetogenic bacteria described previously.