Open AccessDNA from Serratia marcescens confers a hydrophobic character in Escherichia coli
Author(s) -
NessGreenstein Ronit Bar,
Rosenberg Mel,
Doyle R.J.,
Kaplan Nachum
Publication year - 1995
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1995.tb07337.x
Subject(s) - serratia marcescens , escherichia coli , adhesion , ammonium sulfate , dna , hexadecane , enterobacteriaceae , microbiology and biotechnology , blot , chemistry , biology , bacteria , biochemistry , chromatography , gene , genetics , organic chemistry
In order to determine whether hydrophobic surface properties of Serratia marcescens can be transferred to Escherichia coli , E. coli DH5α cells were transformed by DNA fragments from S. marcescens RZ. Fifteen‐hundred E. coli transformants were screened for adhesion to hexadecane and polystyrene. One transformant exhibited increased adhesion to hexadecane droplets, as well as altered kinetics of aggregation in the presence of ammonium sulfate. Western colony blotting revealed that antibodies raised against S. marcescens RZ recognized components) on the transformant outer surface.