Open AccessThe use of arbitrarily primed PCR (AP‐PCR) to develop taxa specific DNA probes of known sequence
Author(s) -
MartínezMurcia A.J.,
RodríguezValera F.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07295.x
Subject(s) - biology , polymerase chain reaction , dna , oligonucleotide , rapd , dna sequencing , taxon , genetics , computational biology , microbiology and biotechnology , gene , botany , population , genetic diversity , demography , sociology
A rapid polymerase chain reaction (PCR) method to obtain taxa‐specific DNA probes has been developed. The oligonucleotide probes derived from the sequences of species‐specific (or other taxa) random amplified polymorphic DNA (RAPD) fragments. The methodology was applied to design probes for the halophilic archael species Haloferax mediterranei . With this technique, DNA probes of known sequence can be generated easily and without any previous knowledge about the properties of the microorganisms.