Open AccessCloning and expression in Escherichia coli of the gene encoding a novel L‐2,4‐diaminobutyrate decarboxylase of Acinetobacter baumannii
Author(s) -
Ikai Hisato,
Yamamoto Shigeo
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07288.x
Subject(s) - subcloning , ecori , biology , southern blot , gene , molecular cloning , microbiology and biotechnology , acinetobacter baumannii , cloning (programming) , escherichia coli , restriction enzyme , restriction map , genetics , gene expression , plasmid , bacteria , computer science , pseudomonas aeruginosa , programming language
The gene encoding L‐2,4‐diaminobutyrate decarboxylase (DABA DC) was cloned from Acinetobacter baumannii ATCC 19606. The gene was evidently under the control of its own promoter. Interestingly, the host carrying this clone also produced an appreciable amount of 1,3‐diaminopropane. Restriction mapping and subsequent subcloning of the cloned insert localized the DABA DC gene within a 2.45‐kb SphI/Eco RI fragment. For endogenous production of DAP, a 1.75‐kb Eco RI/ Pst I region downstream from the DABA DC gene was further required. Southern blot hybridization revealed some heterogeneity in the DABA DC genes among other Acinetobacter species.