Open AccessMutation in dipZ leads to reduced production of active human placental alkaline phosphatase in Escherichia coli
Author(s) -
Beck Reinhard,
Crooke Helen,
Jarsch Michael,
Cole Jeff,
Burtscher Helmut
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07286.x
Subject(s) - escherichia coli , alkaline phosphatase , mutant , phosphatase , placental alkaline phosphatase , biology , enterobacteriaceae , microbiology and biotechnology , biochemistry , mutation , gene , biogenesis , strain (injury) , chemistry , enzyme , anatomy
We have tested the expression of alkaline phosphatases in a mutant strain of Escherichia coli deficient in dipZ , a gene coding for a protein involved in cytochrome c biogenesis, and the isogenic wild‐type strain. The yield of soluble and active human placental alkaline phosphatase was significantly reduced in the mutant but could be fully recovered by expression of dipZ subcloned in a vector with low copy number. Overexpression of E. coli alkaline phosphatase was unaffected in the mutant with or without dipZ co‐expression.