Open AccessCoordination of germ tube formation and surface antigen expression in Candida albicans
Author(s) -
Chaturvedi Vishnu P.,
Vanegas Ricardo,
Chaffin W. LaJean
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07268.x
Subject(s) - candida albicans , germ tube , microbiology and biotechnology , expression (computer science) , germ , antigen , biology , chemistry , genetics , computer science , programming language
If the determinants of shape and cell wall topography are independently regulated and induced in germ tube formation in Candida albicans , these processes may be separable in a non‐germ tube forming strain. The expression of several preferentially expressed hyphal surface components in a parental, non‐germ tube forming variant, and a germ tube forming revertant strain were examined by indirect immunofluorescence. The proportion of germ tubes expressing the determinants and the morphological localization of expression was similar. Few yeast cells in germ tube cultures bound probes and there was no increase in binding by yeast cells of the variant strain. Extraction with β‐mercaptoethanol prior to analysis had little effect on probe binding and the shape of yeast cells were similar. These observations suggest the ability to promote apical expansion in germ tube formation and surface expression of certain markers were coordinately regulated.