Open AccessCharacterization of the aac(6′)‐Ik gene of Acinetobacter sp. 6
Author(s) -
Rudant Eric,
Bourlioux Pierre,
Courvalin Patrice,
Lambert Thierry
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07260.x
Subject(s) - gene , acinetobacter , genetics , biology , genomic dna , dna sequencing , dna , bacteria
The aac(6′)‐Ik gene which confers resistance to aminoglycosides in Acinetobacter sp. 6 CIP A165 was characterized. The resistance gene was identified as a coding sequence of 438 bases pairs corresponding to a protein with a calculated mass of 16627 Da. Alignment of aac(6′)‐Ik with aac(6′)‐Ig from Acinetobacter haemolyticus indicated 83% identity. Like aac(6′)‐Ig of A. haemolyticus and aac(6′)‐Ij of Acinetobacter sp. 13, the aac(6′)‐Ik gene was apparently located in the chromosome and was species specific. The high degree of identity between aac(6′)‐Ig and ‐Ik , compared with genomic DNA relationships between the host species, indicated that these genes have diverged from a common ancestor in a parental Acinetobacter species.