Open AccessMembrane lipids of Mycoplasma fermentans
Author(s) -
Salman M.,
Deutsch I.,
Tarshis M.,
Naot Y.,
Rottem S.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07233.x
Subject(s) - chemistry , mycoplasma , microbiology and biotechnology , membrane , biology , biochemistry
Membranes of Mycoplasma fermentans , incognitus strain, were isolated by a combination of osmotic lysis and sonication. Analysis of membrane lipids revealed, in addition to free and esterified cholesterol, six major polar lipids dominated by a de novo synthesized compound (compound X), which accounts for 64% of the total lipid phosphorus. Compound X was labeled by palmitate, but not by oleate. Mass spectrometry and gas liquid chromatography analyses of compound X revealed two molecular species with molecular masses of 1048 and 1076 representing, a dipalmitoyl‐ and a stearoyl‐palmitoyl‐glycerodiphosphatidylcholine. Compound X has the ability to stimulate human monocytes to secret TNFα and to enhance the fusion of small unilamellar vesicles with MOLT‐3 lymphocytes.