Open AccessSizing the Fusobacterium nucleatum genome by pulsed‐field gel electrophoresis
Author(s) -
Bolstad Anne Isine
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07214.x
Subject(s) - fusobacterium nucleatum , pulsed field gel electrophoresis , xhoi , restriction enzyme , biology , gel electrophoresis , genome , agarose gel electrophoresis , restriction fragment , agarose , microbiology and biotechnology , dna , genetics , bacteria , gene , bamhi , genotype , porphyromonas gingivalis
The genome sizes of Fusobacterium nucleatum strains F1, F3, F6, ATCC 10953, ATCC 25586 and Fev1 were determined by pulsed‐field gel electrophoresis (PFGE). The restriction enzymes Sma I, Sac II, Sal I and Xho I were found to generate a reasonable number of DNA fragments which could be separated by PFGE in agarose gels. The apparent chromosomal lengths of the F. nucleatum strains were determined to be approximately 2.4 million base pairs. This was within the size‐range found by experiments exploiting renaturation kinetics.