Open AccessNutritional upshift response of ribosomal protein gene transcription in Saccharomyces cerevisiae
Author(s) -
Griffioen Gerard,
Mager Willem H.,
Planta Rudi J.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07213.x
Subject(s) - saccharomyces cerevisiae , ribosomal protein , biochemistry , protein kinase a , biology , yeast , glycolysis , gene , kinase , metabolism , rna , ribosome
Switching Saccharomyces cerevisiae from non‐fermentative to fermentative growth by adding glucose to a medium with glycerol as the sole carbon source, leads to a sudden increase in the rate of ribosomal protein gene transcription. By analyzing the nutritional shift response in a variety of yeast mutants and in the presence of different drugs, evidence was obtained that: (i) no de novo protein synthesis is required for this response; (ii) protein kinase A is essential, though independent of intracellular levels of cAMP, whereas protein kinase C is not involved; (iii) proper regulation of sugar phosphorylation is essential; (iv) glycolysis is required for the long term effect of the nutritional upshift; and (v) pathways leading to glucose‐induced activation differ from those leading to gene repression, probably already at the level of glucose transport.