Open AccessCloning and DNA sequence analysis of bmpC , a gene encoding a potential membrane lipoprotein of Borrelia burgdorferi
Author(s) -
Aron Lieselotte,
Alekshun Michael,
Perlee Lorah,
Schwartz Ira,
Godfrey Henry P.,
Cabello Felipe C.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07204.x
Subject(s) - borrelia burgdorferi , biology , open reading frame , immunoscreening , sequence analysis , microbiology and biotechnology , nucleic acid sequence , gene , genetics , peptide sequence , ribosomal binding site , ribosome , cdna library , antibody , rna
Immunoscreening of a λgt11 genomic library of Borrelia burgdorferi expressed in Escherichia coli permitted detection of a clone containing a partial sequence of a B. burgdorferi gene encoding a protein with significant homology to TmpC of Treponema pallidum . Subsequent cloning and DNA sequence analysis revealed an open reading frame encoding a protein with 353 amino acid residues. The open reading frame is preceded by putative promoter sequences and a ribosome binding site, and is initiated with a TTG. The putative protein shares 26% identity with TmpC, contains a signal peptidase II sequence, and is also homologous to the gene products of the recently described bmpA and bmpB of B. burgdorferi . This gene has been designated bmpC . Additional sequencing and restriction analysis indicate that it is located at approximately 400 kbp on the chromosomal map of B. burgdorferi , immediately upstream of bmpA and bmpB .