Open AccessGenetic evidence that hep A gene is involved in the normal deposition of the envelope of both heterocysts and akinetes in Anabaena variabilis ATCC 29413
Author(s) -
Leganés F.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07202.x
Subject(s) - heterocyst , anabaena variabilis , anabaena , biology , microbiology and biotechnology , gene , hepa , plasmid , cyanobacteria , biochemistry , genetics , bacteria , filter (signal processing) , computer science , computer vision
The hep A gene in Anabaena sp. strain PCC 7120 is required for normal formation of the polysaccharide layer of the heterocyst envelope. A plasmid bearing hep A, interrupted by a neomycin‐resistance cassette, was transferred by conjugation to wild type Anabaena variabilis ATCC 29413, so that the interrupted hep A gene replaced a homologous sequence. In the recombinant exconjugants, the envelopes of akinetes as well as of heterocysts were altered.