Open AccessMolecular cloning and sequence analysis of the proBA operon from an extremely thermophilic eubacterium Thermus thermophilus
Author(s) -
Kosuge Takehide,
Tabata Kazuyuki,
Hoshino Takayuki
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07201.x
Subject(s) - thermus thermophilus , operon , gene , biology , nucleic acid sequence , start codon , gene product , genetics , escherichia coli , thermus , codon usage bias , microbiology and biotechnology , thermophile , nucleotide , gene expression , genome , bacteria
A 3.6 kb DNA fragment carrying the Thermus thermophilus proBA region, which encodes the first two steps in the proline biosynthetic pathway, was cloned from the Thermus thermophilus gene library, and its complete nucleotide sequence was determined. The deduced amino acid sequence of γ‐glutamyl kinase (40,657 Da), the product of proB gene, and γ‐glutamyl phosphate reductase (48, 747 Da), the product of proA gene, showed 44.1% and 44.4% identity to those of Escherichia coli , respectively. The termination codon of the proB gene and the initiation codon of the proA gene overlapped by 2 bp. A possible transcriptional termination structure was found downstream of the proA gene but not downstream of the proB gene. These results indicate that the proBA genes of T. thermophilus from a single operon as in E. coli .