Open AccessEvidence for the location of OprM in the Pseudomonas aeruginosa outer membrane
Author(s) -
Gotoh Naomasa,
Itoh Nobuko,
Yamada Hiroshi,
Nishino Takeshi
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07185.x
Subject(s) - pseudomonas aeruginosa , bacterial outer membrane , antiserum , polyclonal antibodies , differential centrifugation , centrifugation , microbiology and biotechnology , membrane , biology , cell membrane , pseudomonadaceae , chemistry , bacteria , escherichia coli , biochemistry , antibody , genetics , gene
OprM with a M r of 49 K is associated with the multidrug resistance of Pseudomonas aeruginosa . Detergent fractionation of bacterial cells has demonstrated that OprM is located in the outer membrane from which it sediments with the other major outer membrane proteins. In this study we have determined the location of OprM as the P. aeruginosa outer membrane. Western immunoblots of cell fractions, obtained by sucrose density gradient centrifugation of whole cell lysates, were probed with an OprM‐specific murine polyclonal antiserum.