Open AccessCytotoxin‐converting phages, φCTX and PS21, are R pyocin‐related phages
Author(s) -
Hayashi Tetsuya,
Matsumoto Hideki,
Ohnishi Makoto,
Yokota Shinichi,
Shinomiya Tomoyuki,
Kageyama Makoto,
Terawaki Yoshiro
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07174.x
Subject(s) - microbiology and biotechnology , bacteriophage , biology , lysogenic cycle , virology , chemistry , genetics , gene , escherichia coli
φCTX is a temperate phage of Pseudomonas aeruginosa harbouring the ctx gene that encodes cytotoxin (CTX). We identified φCTX as an R pyocin‐related phage, by serological and molecular analysis, based on the findings that the infectivity of the phage was inhibited with the antisera directed R pyocins and R pyocin‐related phages and that the φCTX genome showed DNA homology to the genome of PS17 (a representative of the R pyocin‐related phages) as well as to the pyocin R2 genes. Another new CTX‐converting, R pyocin‐related phage named PS21 was isolated from a CTX‐producing strain of P. aeruginosa , suggesting the distribution of the ctx gene by certain members of R pyocin‐related phage family.