Open AccessThe restriction endonuclease Rfl FII, isolated from Ruminococcus flavefaciens FD‐1, recognizes the sequence 5′‐AGTACT‐3′, and is inhibited by site‐specific adenine methylation
Author(s) -
Morrison Mark,
Mackie Roderick I.,
White Bryan A.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07162.x
Subject(s) - restriction enzyme , biology , ruminococcus , dna , microbiology and biotechnology , methylation , endonuclease , recognition sequence , biochemistry , genetics , bacteria
Molecular studies of the rumen bacterium Ruminococcus flavefaciens are constrained by the lack of stable gene transfer systems. We report here on the characterization of Rfl FII, a restriction endonuclease isolated from R. flavefaciens FD‐1. The enzyme is an isoschizomer of Sca I, and cleavage of the DNA is blunt‐ended, between the internal TA dinucleotide sequence of 5′‐AGTACT‐3′. Chromosomal DNA preparations were used to demonstrate that adenine methylation of DNA within the sequence 5′‐GTAC‐3′ inhibits both Rfl FII and the restriction endonucleases Rsa I and Sca I. Chromosomal DNA from R. flavefaciens FD‐1 is also host modified to protect against cleavage by Sca I.