Open AccessCloning and expression of the plasmid encoded β‐d‐galactosidase gene from a Lactobacillus plantarum strain of dairy origin
Author(s) -
Mayo Baltasar,
González Beatriz,
Arca Pilar,
Suárez Juan Evaristo
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07157.x
Subject(s) - plasmid , cloning (programming) , lactobacillus plantarum , strain (injury) , gene , biology , microbiology and biotechnology , molecular cloning , bacteria , genetics , gene expression , lactic acid , anatomy , computer science , programming language
The β‐galactosidase (β‐Gal) gene from Lactobacillus plantarum C3.8 was cloned and expressed in Lactococcus lactis and Escherichia coli . Hybridization experiments indicated that the gene is located on a plasmid and is present in other strains of Lactobacillus plantarum . Its sequence is very similar to a Leuconostoc lactis β‐Gal gene. Expression of the gene, both in Lactobacillus plantarum and in Lactococcus lactis , was four‐fold higher in cells grown in lactose compared to those grown in glucose. The presence of the β‐Gal gene in Lactococcus lactis allowed this bacterium to be efficient in clotting milk.