Open AccessAerobic biodegradation of 3‐aminobenzoate by Gram‐negative bacteria involves intermediate formation of 5‐aminosalicylate as ring‐cleavage substrate
Author(s) -
Russ Rainer,
Müller Claudia,
Knackmuss HansJoachim,
Stolz Andreas
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07156.x
Subject(s) - chemistry , strain (injury) , bacteria , biodegradation , biology , organic chemistry , genetics , anatomy
The aerobic metabolism of 3‐aminobenzoate by bacteria was studied. Bacterial strains degrading 3‐aminobenzoate were obtained by enrichment with 3‐aminobenzoate (strain Ia3) or 5‐aminosalicylate (strains BN9 and 5AS1). During growth with 3‐aminobenzoate, strain Ia3 and strain 5AS1 transiently accumulated 5‐aminosalicylate in the culture broth. In the presence of inhibitors of 5‐aminosalicylate 1,2‐dioxygenase, resting cells of all three strains converted 3‐aminobenzoate to stoichiometric amounts of 5‐aminosalicylate. 5‐Aminosalicylate 1,2‐dioxygenase activity was induced in all strains after growth with 3‐aminobenzoate or 5‐aminosalicylate, but not after growth in complex media.