Open AccessDetermination and comparison of Lactobacillus delbrueckii ssp. lactis DSM7290 promoter sequences
Author(s) -
Tiberius Matern Hugo,
Robert Klein Jürgen,
Henrich Bernhard,
Plapp Roland
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07154.x
Subject(s) - promoter , biology , consensus sequence , primer extension , ribosome , bacillus subtilis , lactobacillus , homology (biology) , escherichia coli , gene , transcription (linguistics) , genetics , upstream activating sequence , start codon , shine dalgarno sequence , ribosomal rna , 16s ribosomal rna , microbiology and biotechnology , nucleotide , rna , bacteria , peptide sequence , gene expression , linguistics , philosophy
The transcriptional start points of ten Lactobacillus delbrückii ssp. lactis DSM7290 genes were determined by primer extension. The upstream located promoter regions, including potential −35 and −10 regions and the spacing between them were compared to the well‐known Escherichia coli and Bacillus subtilis promoters. The Lb. delbrückii −35 consensus sequence (TTGACA) seems to be less conserved then the E. coli sequence. The nucleotides TGC were often found upstream of the −10 region (TATAAT). The most frequently observed spacing between the two core promoter regions was 17 nt and the main distance between the −10 region and the transcriptional start point was mostly determined to be 6 nt in contrast to 7 nt, as described for E. coli promoters. The preferred initiation nucleotides in Lb. delbrückii were shown to be definitely purines (A or G). The ribosome binding sites located downstream of the promoters revealed the consensus sequence 3′‐UCCUCCU‐5′, being the predicted 3′‐OH end of the Lactobacillus 16S rRNA with a high degree of homology to known 16S rRNAs.