Open AccessVariable expression of O‐antigen and the role of lipopolysaccharide as an adhesin in Aeromonas sobria
Author(s) -
Francki Kathy T.,
Chang Barbara J.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07150.x
Subject(s) - lipopolysaccharide , bacterial adhesin , microbiology and biotechnology , antigen , strain (injury) , blot , aeromonas , chemistry , enterobacteriaceae , monoclonal antibody , bacteria , escherichia coli , biology , antibody , biochemistry , gene , immunology , genetics , anatomy
On initial isolation of Aeromonas sobria 3767 from a diarrhoeal stool specimen, two colony types were obtained: opaque (3767O) and translucent (3767T). Strain 3767O consistently produced lipopolysaccharide (LPS) core and O‐antigen side chain, detectable by SDS‐PAGE and by Western blotting with an O‐antigen‐specific monoclonal antibody. Strain 3767T produced LPS core but the amount of O‐antigen was dependent on factors including growth medium and bacterial growth phase. Strain 3767T exhibited significantly lower levels of adhesion to HEp‐2 cells than 3767O and this correlated with the level of LPS expression, with the greatest reduction (61%) at stationary phase when no LPS was detectable. The results implicate LPS as an adhesin for A. sobria 3767.