Open AccessHigh pressure conditions stimulate expression of chloramphenicol acetyltransferase regulated by the lac promoter in Escherichia coli
Author(s) -
Kato Chiaki,
Sato Takako,
Smorawinska Maria,
Horikoshi Koki
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07149.x
Subject(s) - chloramphenicol acetyltransferase , escherichia coli , chloramphenicol , acetyltransferase , lac operon , microbiology and biotechnology , chemistry , biology , gene expression , promoter , biochemistry , antibiotics , gene , acetylation
Recombinant plasmids with the chloramphenicol acetyltransferase (CAT) structural gene behind several kinds of promoters were tested for expression in Escherichia coli during growth at atmospheric pressure (0.1 MPa) and at high pressure (30 MPa). Expression of the CAT gene from the lac promoter was remarkably activated (approx. 78‐fold) by high pressure in the absence of the inducer isopropyl‐β‐d‐thiogalactopyranoside (IPTG). The stimulation of the CAT activity by the lac promoter at high pressure did not simply result from an increased plasmid copy number, because the CAT activities from the other promoters and β‐lactamase activities were unaffected at high pressure.