Open AccessDetection of stress proteins in Porphyromonas gingivalis and other oral bacteria by Western immunoblotting analysis
Author(s) -
Vayssier C.,
Mayrand D.,
Grenier D.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07117.x
Subject(s) - porphyromonas gingivalis , hsp60 , polyclonal antibodies , virulence , microbiology and biotechnology , heat shock protein , groel , monoclonal antibody , bacteroidaceae , biology , bacteria , antibody , hsp70 , escherichia coli , biochemistry , immunology , genetics , gene
Detection of stress proteins in Porphyromonas gingivalis was investigated by SDS‐PAGE and Western immunoblotting procedure using a polyclonal antibody (anti hsp60) and a monoclonal antibody (anti‐Dnak). Results indicate that P. gingivalis can elicit a hsp60‐like stress protein when submitted to different environmental stresses such as a heat shift from 35°C to 43°C, a pH drop from 7.2 to 6.0 or an increase in oxygen concentration. Virulent and non‐virulent strains of P. gingivalis responded the same way. The other bacterial species tested also showed an increased synthesis of a GroEL‐like protein after heat shock, as detected by the anti hsp60 antibody. However, the monoclonal anti‐Dnak recognized an hsp70‐like protein only in two of the tested species.