Open AccessUse of Yarrowia lipolytica hexokinase for the quantitative determination of trehalose 6‐phosphate
Author(s) -
Blázquez Miguel A.,
Gancedo Juana M.,
Gancedo Carlos
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07102.x
Subject(s) - yarrowia , trehalose , schizosaccharomyces pombe , yeast , hexokinase , biochemistry , saccharomyces cerevisiae , phosphate , mutant , chemistry , wild type , biology , enzyme , glycolysis , gene
This paper describes a procedure for the quantitative determination of trehalose 6‐phosphate (T6P) based on its ability to inhibit hexokinase from Yarrowia lipolytica . The assay is linear between 1 nmol and at least 8 nmol. The concentration of T6P in wild‐type Saccharomyces cerevisiae (0.15 mM) and in ras2 mutants (0.25 mM) remained unchanged in the exponential or stationary phase of growth or after heat shock. A tps1 mutant affected in T6P synthase did not show detectable T6P. Heat shock increased the concentration of T6P in Schizosacharomyces pombe from 0.43 to 0.75 mM.