Open AccessIndividualisation of two new genomic groups among American Borrelia burgdorferi sensu lato strains
Author(s) -
Assous Marc Victor,
Postic Danièle,
Paul Gérard,
Névot Pierre,
Baranton Guy
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07081.x
Subject(s) - borrelia burgdorferi , sensu , biology , amplicon , typing , southern blot , borrelia , virology , genomic dna , microbiology and biotechnology , polymerase chain reaction , gene , genetics , antibody , botany , genus
We developed a quick typing method for Borrelia burgdorferi sensu lato strains using a fla gene‐based PCR assay, followed by dot blot hybridization with non‐radioactive species‐specific probes. Thirty‐six out of 46 strains belonged to one of the four described species ( B. burgdorferi sensu stricto n = 11, B. garinii n = 11, B. afzelii n = 9 and B. japonica n = 5) and hybridized with its own species‐specific probe. Among the 10 remaining American strains, two new additional genomic groups were identified. This finding was confirmed by direct sequenching of the fla gene‐derived amplicons and whole DNA hybridization.