Open AccessRibosomal RNA gene ( rrn ) organization in enterococci
Author(s) -
Sechi Leonardo A.,
Zuccon Fabio M.,
Mortensen Joel E.,
DaneoMoore Lolita
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07051.x
Subject(s) - ecori , ribosomal rna , biology , 23s ribosomal rna , restriction enzyme , enterococcus hirae , operon , rna , microbiology and biotechnology , genetics , 50s , ribosomal dna , enterococcus , gene , ribosome , escherichia coli , bacteria , phylogenetics
A cloned 1.8‐kb probe containing the 3′ end of 16S ribosomal RNA and the 5′ end of 23S ribosomal RNA from Enterococcus hirae was used to analyze various endonuclease digests of enterococci. In the ATCC strains tested we observed a remarkable conservation of the Apa I sites in the rrn operons, and a partial conservation of Eco RI sites. Using a number of other endonuclease digestions with the Apa I rrn probe, we estimate the number of rrn operons in enterococci to be between five and six.