Open AccessAn agar‐overlay method for detection of toxins produced by Escherichia coli
Author(s) -
Parreira Valéria Regina,
Arns Clarice Weis,
Yano Tomomasa
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07050.x
Subject(s) - enterotoxin , agar , escherichia coli , hela , microbiology and biotechnology , vero cell , chemistry , biology , bacteria , virology , cell , biochemistry , gene , virus , genetics
An agar overlay method, with Vero and Hela cells, was used for detection of heat‐labile enterotoxin and verotoxin from Escherichia coli . The method is more sensitive than the conventional cell culture assay, is rapid, easy to perform, and is suitable for epidemiological studies.