Open AccessElectron transfer reactions for the reduction of NADP + in Methanosphaera stadtmanae
Author(s) -
Wong Denny,
Lin Zhaosheng,
Juck David F.,
Terrick Karin A.,
Sparling Richard
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07047.x
Subject(s) - hydrogenase , oxidoreductase , cofactor , electron transfer , enzyme , electron transport chain , chemistry , nad+ kinase , methanol , biochemistry , alcohol dehydrogenase , photochemistry , organic chemistry
Methanosphaera stadtmanae , a member of the Methanobacteriales reduces methanol, but not CO 2 with H 2 or 2‐propanol to produce methane. In cell‐free extracts of M. stadtmanae the activities of several enzymes involved in electron transfer were measured. The activities of an F 420 ‐nonreactive hydrogenase, NADP + : F 420 oxidoreductase, NADP + ‐dependent 2‐propanol dehydrogenase, and a methyl viologen dependent F 420 dehydrogenase were observed. Based on the presence of these particular enzyme activities, their cofactor requirements and the absence of F 420 ‐dependent hydrogenase activity, a model of the electron transport pathway through the coenzyme F 420 to provide electrons for biosynthesis, was formulated.