Open AccessIdentification and nucleotide sequence of Brucella melitensis L7/L12 ribosomal protein
Author(s) -
Bachrach Gilad,
BarNir Dror,
Banai Menachem,
Bercovier Herve
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07039.x
Subject(s) - brucella melitensis , ribosomal protein , biology , nucleic acid sequence , gene , ribosomal rna , genetics , protein sequencing , brucella , sequence analysis , peptide sequence , microbiology and biotechnology , virology , ribosome , rna , brucellosis
DNA sequencing of the gene encoding a Brucella melitensis 12‐kDa protein revealed that this protein was the ribosomal protein L7/L12. The B. melitensis L7/L12 DNA sequence was identical to that of the corresponding B. abortus gene, showing the near identity of these two organisms. When comparing the sequence of this protein to that of other organisms some domains were highly conserved, especially the C‐terminus, which contrasted with the lack of conservation of the sequences at the N‐terminus. The finding that the ribosomal protein L7/L12 of Brucella is an immunodominant antigen provides a new rationale to explain the activity of ribosomal vaccines.