Open AccessDipeptidyl aminopeptidase yspI mutants of Schizosaccharomyces pombe : Genetic mapping of dpa 1 + on chromosome III
Author(s) -
Villa Lourdes,
SuárezRendueles Paz
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07033.x
Subject(s) - schizosaccharomyces pombe , mutant , aminopeptidase , biology , gene , microbiology and biotechnology , chromosome , mutation , genetics , biochemistry , amino acid , leucine
A mutant strain of Schizosaccharomyces pombe lacking dipeptidyl aminopeptidase yspI was isolated from a strain already defective in aminopeptidase activity by means of a staining technique with the chromogenic substrate ala‐pro‐4‐methoxy‐β‐naphthylamide to screen colonies for the absence of the enzyme. The defect segregated 2 + :2 − in meiotic tetrads, indicating a single chromosomal gene mutation, which was shown to be recessive. Gene dosage experiments indicated that the mutation resides in the structural gene of dipeptidyl aminopeptidase yspI, dpa 1 + . The dpa 1 + gene was located on chromosome III by using l m‐ fluorophen‐ylalanine‐induced haploidization and mitotic analysis. dpa1 mutants did not show any obvious phenotype under a variety of conditions tested.