Open AccessCorrelation between cell‐associated mannose‐sensitive hemagglutination by Vibrio parahaemolyticus and adherence to a human colonic cell line Caco‐2
Author(s) -
Nagayama Kenichi,
Oguchi Tomiaki,
Arita Michiko,
Honda Takeshi
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07032.x
Subject(s) - vibrio parahaemolyticus , hemagglutination , mannose , microbiology and biotechnology , biology , hemagglutination assay , caco 2 , cell culture , bacteria , virology , biochemistry , virus , genetics , titer
Cell‐associated hemagglutination (cHA) activity with human erythrocytes was examined for 468 clinical and 71 environmental strains of Vibrio parahaemolyticus . Approximately 95% of the strains tested were cHA positive irrespective of source or Kanagawa phenomenon. 75% of clinical strains showed relatively strong mannose‐sensitive hemagglutination (MSHA), whereas 88% of the environmental strains showed relatively weak mannose‐resistant hemagglutination (MRHA). Adherence of V. parahaemolyticus to Caco‐2 cells was also determined. A clear positive correlation between cell‐associated MSHA and adherence to Caco‐2 cells was observed.