Open AccessCalvin cycle genes in Nitrobacter vulgaris T3
Author(s) -
Strecker Maren,
Sickinger Eva,
English Robert S.,
Shively Jessup M.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb07005.x
Subject(s) - biology , biochemistry , rubisco , ribulose , aldolase a , gene cluster , gene , genetics , enzyme
The genes encoding the Calvin cycle enzymes of Nitrobacter vulgaris T3 are found as two separate clusters on the chromosome. One cluster contains the genes for the large and small subunits of ribulose‐1,5‐biphosphate carboxylase/oxygenase (RuBisCO), glyceraldehyde‐3‐phosphate dehydrogenase, and one encoding a regulatory protein of the LysR family. The other cluster contains the genes for fructose‐1,6‐/sedoheptulose‐1,7‐bisphosphatase, phosphoribulokinase, and fructose‐1,6‐/sedoheptulose‐1,7‐biphosphate aldolase. With the exception of the LysR‐like gene, the genes in each cluster are apparently transcribed in the same direction. The deduced amino acid sequence of both the large and small subunits of RuBisCO are most similar (84–86%) to those of Thiobacillus ferrooxidans and Chromatium vinosum . The deduced sequences of phosphoribulokinase and fructose/sedoheptulose bisphosphatase are 67–73 aand 44–46% similar to those reported for other autotrophic bacteria, respectively.