Open AccessOverexpression, phosphorylation, and growth effects of ORF162, a Klebsiella pneumoniae protein that is encoded by a gene linked to rpoN , the gene encoding δ 54
Author(s) -
Begley Gail S,
Jacobson Gary R
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06918.x
Subject(s) - gene product , klebsiella pneumoniae , pep group translocation , biology , escherichia coli , gene , mutant , phosphorylation , phosphoenolpyruvate carboxykinase , transcription (linguistics) , regulator gene , microbiology and biotechnology , bacteria , bacillus subtilis , biochemistry , regulation of gene expression , genetics , gene expression , linguistics , philosophy
The product of a Klebsiella pneumoniae gene, orf162 , may regulate δ 54 ‐dependent transcription and has sequence similarity to proteins of the phosphoenolpyruvate‐dependent phosphotransferase system (PTS). We have overproduced the product of orf162 and demonstrated its PTS‐dependent phosphorylation in Escherichia coli extracts. We have also observed moderate growth inhibition of a wild‐type, but not a δ 54 ‐mutant, strain by overexpression of orf162 . These results are consistent with the hypothesis that the product of orf162 could be a regulatory link between the PTS and δ 54 activity in bacteria.