Open AccessNucleotide sequence and analysis of the gene in Borrelia burgdorferi encoding the immunogenic P39 antigen
Author(s) -
Simpson Warren J.,
Cieplak Witold,
Schrumpf Merry E.,
Barbour Alan G.,
Schwan Tom G.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06917.x
Subject(s) - borrelia burgdorferi , open reading frame , biology , nucleic acid sequence , gene , treponema , genetics , peptide sequence , sequence analysis , spirochaetaceae , microbiology and biotechnology , virology , antibody , syphilis , human immunodeficiency virus (hiv)
The P39 antigen is a specific, highly conserved, and immunogenic protein of Lyne disease spirochetes, Borrelia burgdorferi sensu lato. The nucleotide sequence of the gene encoding this protein was determined and found to be the first of two tandemly arranged open reading frames located on the spirochete's chromosome. These two open reading frames were designated bmpA for the gene encoding P39 and bmpB for the gene encoding the putative protein ORF2 encoded by the second open reading frame. The nucleic acid sequence identity for the two open reading frames was 62% while their deduced amino acid sequences were 52% identical. Comparison to sequence data bases demonstrated that the deduced amino acid sequences of both P39 and ORF2 were homologous to TmpC, a putative outer or cytoplasmic membrane lipoprotein of the syphilis spirochete, Treponema pallidum .