Open AccessAnaerobic respiration of Shewanella putrefaciens requires both chromosomal and plasmid‐borne genes
Author(s) -
Saffarini D.A.,
DiChristina T.J.,
Bermudes D.,
Nealson K.H.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06900.x
Subject(s) - shewanella putrefaciens , complementation , plasmid , mutant , biology , shewanella , gene , dna , microbiology and biotechnology , anaerobic respiration , genetics , bacteria
Pleiotropic respiratory mutants, incapable of growth on any electron acceptor other than oxygen, were isolated from two strains of Shewanella putrefaciens (MR‐1 and sp200). All anaerobic respiratory functions were restored by complementation of the mutants with specific cloned DNA fragments. Southern hybridization experiments revealed that the fragment that complements the MR‐1 mutant was localized on the megaplasmids of both strains, while the fragment that complements the sp200 mutant was chromosomal. Neither of these fragments hybridized with the anaerobic regulatory genes of S. putrefaciens ( etrA ) or E. coli ( fnr ).