Open AccessSubdivision of Salmonella enteritidis PT 4 by pulsed‐field gel electrophoresis: Potential for epidemiological surveillance
Author(s) -
Powell N.G.,
Threlfall E.J.,
Chart H.,
Rowe B.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06888.x
Subject(s) - salmonella enteritidis , pulsed field gel electrophoresis , salmonella , microbiology and biotechnology , epidemiological surveillance , epidemiology , biology , medicine , bacteria , genetics , gene , genotype
Using pulsed‐field gel electrophoresis (PFGE), nine profile types were identified in Xba I digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to pulsed‐field profile (PFP) 1 predominated. DNA‐DNA hybridization studies demonstrated that a band of approximately 57 kb was associated with the presence of the 38 MDa S. enteritidis ‘serovar‐specific’ plasmid. It is concluded that PFGE provides a method for discriminating strains of S. enteritidis PT 4 suitable for epidemiological investigations.