Open AccessGenotypic and phenotypic relatedness of 80 strains of Branhamella catarrhalis of worldwide origin
Author(s) -
Christensen Jens J?rgen,
Ursing Jan,
Bruun Brita
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06882.x
Subject(s) - moraxella catarrhalis , moraxella (branhamella) catarrhalis , neisseria , microbiology and biotechnology , genotype , neisseriaceae , moraxella , biology , neisseria meningitidis , phenotype , strain (injury) , bacteria , gene , haemophilus influenzae , genetics , antibiotics , anatomy
80 clinical Branhamella catarrhalis strains of worldwide origin were examined for genotypic relatedness and phenotypic characteristics. Using a quantitative bacterial dot method for DNA‐DNA hybridization the strains were found to form a homogeneous group with ΔT m ‐values ranging from 0.0–2.3°C. In Minibact‐N, an identification kit for oxidase positive, Gram‐negative diplococci using eight phenotypic characteristics, all isolates were correctly identified and also demonstrated complete homogeneity except for β‐lactamase production. Type strains representing the genera Branhamella, Moraxella and Neisseria were also examined for comparison. B. catarrhalis strain NCTC 4103‐known to be atypical‐had a ΔT m ‐value of 5.7°C and produced γ‐glutamylaminopeptidase, in contrast to all other B. catarrhalis strains. In GN MicroPlate TM , a kit which tests utilizable carbon sources, B. catarrhalis strains were found to be able to utilize up to 16 to 95 carbon sources.