Open AccessThe phylogenetic position of Hydrogenobacter acidophilus based on 16S rRNA sequence analysis
Author(s) -
Shima Seigo,
Yanagi Miyoko,
Saiki Hiroshi
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06877.x
Subject(s) - phylogenetic tree , 16s ribosomal rna , strain (injury) , biology , phylogenetics , aquifex aeolicus , eubacterium , ribosomal rna , genetics , microbiology and biotechnology , gene , bacteria , escherichia coli , anatomy
Hydrogenobacter acidophilus strain 3H‐1 is a thermoacidophilic, obligately chemolithoautotrophic, hydrogen‐oxidizer isolated from a Japanese solfataric field. Strain 3H‐1 requires elemental sulfur for growth. We used PCR to amplify the 16S rRNA gene of strain 3H‐1, and sequenced the amplification product directly. Phylogenetic analyses show strain 3H‐1 is closely related to Aquifex pyrophilus and may be located in the deepest branch within the eubacterial phylogenetic tree. Sulfur‐dependency of the ancestral eubacterium is also discussed.