Open AccessBiochemical and genetic heterogeneity of staphylococcal protein A
Author(s) -
Sakurada Junji,
Zhijun Li,
Seki Keiko,
Murai Miyo,
Usui Akemi,
Morihigashi Mami,
Jitsukawa Hiroko,
Kyung Seong Hee,
Mutou Mitsuaki,
Masuda Shogo
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06867.x
Subject(s) - ecorv , staphylococcus aureus , polymerase chain reaction , biology , gene , dna , antibody , genetics , microbiology and biotechnology , bacteria , restriction fragment length polymorphism
We investigated the biochemical and genetic heterogeneity of protein A from Staphylococcus aureus . SpA genes ( spas ) of various strains were heterogeneous when detected as Dra I and Eco RV fragments of chromosomal DNA. Polymerase chain reaction using primers to detect DNA encoding the IgG‐binding domains in spa revealed that they numbered between 2 and 5. Protein A from several S. aureus strains showed two types of reactivities to immunoglobulins in normal canine serum according to the number of active domains.