Open AccessThe nusG gene of Streptomyces griseus : Cloning of the gene and analysis of the A‐factor binding properties of the gene product
Author(s) -
Kuberski Sabine,
Kasberg Thomas,
Distler Jürgen
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06863.x
Subject(s) - streptomyces griseus , biology , gene , gene product , nucleic acid sequence , cloning (programming) , genetics , streptomycetaceae , streptomyces , microbiology and biotechnology , gene expression , actinomycetales , bacteria , computer science , programming language
The nusG gene of Streptomyces griseus was cloned and the nucleotide sequence determined. It encodes a protein with an identify of 76% to the reported receptor (VbrA) for VB‐C, an autoregulatory factor in Streptomyces virginae . NusG protein was expressed in Escherichia coli . However, no binding activity for A‐factor, an butyrolactone autoregulator in S. griseus very similar to VB‐C, could be detected. The nusG gene of S. griseus does not seem to encode the A‐factor‐binding protein.