Open AccessEnzymatic desulfurization of dibenzothiophene by a cell‐free system of Rhodococcus erythropolis D‐1
Author(s) -
Ohshiro Takashi,
Hine Yoshimitsu,
Izumi Yoshikazu
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06850.x
Subject(s) - dibenzothiophene , flue gas desulfurization , rhodococcus , chemistry , sulfur , enzyme , rhodococcus rhodochrous , organic chemistry , biochemistry , chromatography
The enzymatic desulfurization of dibenzothiophene (DBT) to 2‐hydroxybiphenyl (2‐HBP) was detected in extracts of Rhodococcus erythropolis D‐1 grown on DBT as a sole source of sulfur. In the dialyzed cell‐free extracts, NADH was absolutely required for the activity. DBT desulfurization proceeded linearly with the reaction time, and stoichiometric amounts of 2‐HBP were finally formed. The activity was stimulated by increasing the protein and NADH concentrations up to 5 mg ml −1 and 5 mM, respectively, when 0.1 mM DBT was present in the reaction mixture.