Open AccessDistribution of glycerol dehydrogenase and glycerol kinase activity in halophilic archaea
Author(s) -
Oren Aharon,
Gurevich Peter
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06846.x
Subject(s) - halophile , glycerol , halobacterium , glycerol kinase , dihydroxyacetone , biochemistry , archaea , dehydrogenase , halobacteriaceae , biology , lactate dehydrogenase , haloarchaea , nad+ kinase , enzyme , chemistry , bacteria , halobacterium salinarum , genetics , gene
A constitutive NAD + ‐dependent glycerol dehydrogenase activity was detected in Halobacterium salinarium and Halobacterium cutirubrum . Optimal activity was found at 3 M KCl and pH 8–10. No glycerol dehydrogenase activity could be demonstrated in representatives of the genera Haloferax and Haloarcula , even when grown in the presence of glycerol, or in Halobacterium saccharovorum and Halobacterium sodomense . Glycerol kinase activity was shown to be present constitutively in all halophilic archaea examined. The finding that glycerol dehydrogenase is found only in part of the halophilic arachaea makes dihydroxyacetone an improbable candidate as the precursor for the glycerol moiety of halobacterial lipids.