Open AccessA murine monoclonal antibody directed against a yeast cell wall glycoprotein antigen of the yeast genus Saccharomyces
Author(s) -
Bröker Michael,
Harthus HansPeter,
Barnes Roger M.R.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06844.x
Subject(s) - monoclonal antibody , yeast , epitope , saccharomyces cerevisiae , glycoprotein , biology , saccharomyces , antigen , cell wall , immunofluorescence , microbiology and biotechnology , antibody , biochemistry , genetics
Murine monoclonal antibodies (mAbs) were selected against a cell wall glycoprotein of Saccharomyces cerevisiae . One of the mAbs (92‐276/018) specifically identified S. cerevisiae and the sibling species S. paradoxus, S. pastorianus and S. bayanus in immunofluorescence studies and immunoblot analyses, while no other yeast genera except Saccharomyces were recognized. Further analysis indicated that the mAb 92‐276/018 reacts with an epitope in the carbohydrate chain of the cell wall glycoproteins.