Open AccessCloning and characterization of the ribosomal RNA gene complex from the plant pathogen Verticillium albo‐atrum
Author(s) -
Griffen Alison M.,
Heale James B.,
Bainbridge Brian W.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06843.x
Subject(s) - biology , verticillium , ribosomal dna , ribosomal rna , genetics , restriction enzyme , genomic dna , gene , spacer dna , microbiology and biotechnology , intergenic region , restriction map , internal transcribed spacer , genome , nucleic acid sequence , botany , phylogenetics
Abstract The DNA coding for the ribosomal RNA gene complex (rDNA) has been cloned from isolate 621P(PV1) of Verticillium albo‐atrum which is pathogenic for hops ( Humulus lupulus ). The rDNA was mapped using a range of restriction enzymes. The functional units of the intergenic spacer (IGS), 18S, 5.8S and 25S regions were located by hybridization to specific gene probes from the rDNA complex of Aspergillus nidulans . The start points of the 18S and 5.8S regions were confirmed by partial sequencing. A genomic restriction enzyme map was found to be identical with the map of the cloned DNA. The rDNA repeat was 7.6 kb in length and this was used as an homologous probe to analyse the size of the repeat in 18 hop isolates of V. albo‐atrum strains and in one isolate from alfalfa (Luc2). All of the isolates had a repeat size of 7.6 kb except for Luc2 where the rDNA complex was 8.4 kb.