Open AccessSequence analysis of cohesive ends of the actinophage RP3 genome and construction of a transducible shuttle vector
Author(s) -
Kinner Elke,
Počta Darko,
Ströer Sandra,
Schmieger Horst
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06842.x
Subject(s) - cosmid , shuttle vector , sequence (biology) , biology , dna , genome , dna sequencing , sequence analysis , genetics , whole genome sequencing , vector (molecular biology) , computational biology , microbiology and biotechnology , gene , recombinant dna
The sequence of the cohesive ends of actinophage RP3 DNA has been determined. As with all other phages of Gram‐positive bacteria that have been studied sofar, RP3 DNA has 3′‐protruding ends. A shuttle cosmid has been constructed containing this cos area which can be efficiently transduced by phage RP3 to host cells of Streptomyces rimosus .