Open AccessQuantitative determination of intracellular depolymerase activity in Pseudomonas oleovorans inclusions containing poly‐3‐hydroxyalkanoates with long alkyl substituents
Author(s) -
Foster L.John R.,
Lenz Robert W.,
Clinton Fuller R.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06841.x
Subject(s) - alkyl , intracellular , chemistry , biochemistry , chromatography , organic chemistry
Research regarding the accurate, quantitative degradation of novel poly‐3‐hydroxyalkanoates has been restricted by the absence of an appropriate monitoring technique. The calibration of a gas chromatograph to poly‐3‐hydroxyalkanoate reveals a linear relationship between the area under gas chromatograph tracings and polymer weight. With this new method, poly‐3‐hydroxyactanoate granules isolated from Pseudomonas oleovorans , which were incubated at 30°C in an alkaline buffer, exhibited a linear degradation rate. Degradation was inhibited by the presence of Triton X‐100 and phenylmethylsulfonyl fluoride. The depolymerase was demonstrated to be associated with the polymer granule complex and most likely possessed serine residues at its active site.