Open AccessExpression of the Streptomyces griseus α‐amylase gene in Escherichia coli
Author(s) -
Vigal T.,
Martin J.F.,
Gil J.A.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06838.x
Subject(s) - streptomyces griseus , escherichia coli , microbiology and biotechnology , lac operon , biology , gene , gene expression , promoter , streptomyces albus , streptomyces , biochemistry , bacteria , genetics
The amy gene of Streptomyces griseus was not expressed in Escherichia coli cells due to the lack of recognition of the amy promoter by the E. coli RNA polymerase, as confirmed by using promoter‐probe vectors. The expression of the amy gene in E. coli was detected only when the promoter‐less gene was placed under the control of the lacZ promoter and was dependent on the level of IPTG added to the medium. The extracellular α‐amylase detected in the culture broth seems to be released by cellular lysis. When the amy gene lacking both leader peptide and promoter was transcribed from the lacZ promoter, no α‐amylase activity was detected but larger E. coli cells and inclusion bodies were observed.